Vim li cas cryovials tawg?Yuav zam tau li cas?

Vim li cas cryovials tawg?Yuav zam tau li cas?

Hauv kev sim, peb tuaj yeem sivcryovialskom khov cov qauv, tab sis thaum khov nrog kua nitrogen,cryovialsfeem ntau tawg, uas tsis tsuas yog ua rau poob ntawm kev sim cov qauv, tab sis kuj tseem yuav ua rau kev puas tsuaj rau cov qauv.Cov kws sim ua rau muaj kev phom sij, yog li yuav ua li cas tiv thaiv qhov no?

Yog vim li cas:

Ua ntej tshaj plaws,cryovialstsis tuaj yeem tso ncaj qha rau hauv cov kua theem ntawm cov kua nitrogen rau kev khaws cia.Vim hais tias cov khoom ntawm lub raj lub cev thiab lub hau ntawm cov khoom sivcryovialstxawv, cov nqi ntawm thermal expansion thiab contraction thaum lub sij hawm khov kuj txawv.Yog koj tso lubcryovialncaj qha rau hauv cov kua theem, koj tuaj yeem tso kua nitrogen ntws mus rau hauv lub raj.Thaum resuscitating tus qauv tom ntej, muab tso raucryogenic vialsrau hauv dej da dej ntawm 37 ° C, cov kua nitrogen hauv lub raj sai sai vaporized thiab nthuav dav, tab sis cov roj tsis tuaj yeem khiav tawm ntawm lub raj hauv lub sijhawm, uas ua rau lub raj cryopreservation tawg.

Yuav zam li cas:

1. Tsis txhob khaws ciacryovialsncaj qha nyob rau hauv cov kua theem, tab sis nyob rau hauv lub gas theem.Los yog cia li khov nws ncaj qha rau hauv lub tub yees.Nco ntsoov tsis txhob tso nws ncaj qha rau hauv qab ntawm cov kua nitrogen.

2. Siv cov kev sib hloov sab hauvcryotubes.

Ntawm chav kawm, txawm internally rotatedcryotubestsis tuaj yeem muab tso ncaj qha rau hauv cov kua theem, tab sis sab hauv tigcryotubesmuaj qhov ua tau zoo ntawm qhov kub thiab txias dua li lub kaus mom sab nraud, uas tuaj yeem txo qhov muaj peev xwm tawg thiab muaj kev nyab xeeb dua.Kev sib hloov sab nraudcryotubeyog tsim los rau cov neeg kho tshuab khov, yog li nws tsis haum rau cov kua nitrogen cia.

3. Yog li koj yuav ua li cas yog tias koj xav tau tiag tiag khaws cia rau hauv cov kua theem?Hauv kev teb rau qhov teeb meem no, muaj tiag tiag lub tes tsho cryopreservation tube tshwj xeeb tsim los rau lub hom phiaj no, uas tuaj yeem siv los kaw lub raj cryopreservation thiab muab tso rau hauv cov kua theem.Tau kawg, koj tuaj yeem siv zaj duab xis sealing, daim kab xev kho mob, thiab lwm yam los kaw nws, thiaj li yuav tsis muaj qhov tawg.