CovELISAphaj yog ib qho cuab yeej tseem ceeb rau ELISA, qhov kev ntsuam xyuas enzyme-txuas immunosorbent.Muaj ntau yam uas cuam tshuam rau kev ua tiav ntawm ELISA kev sim.Xaiv cov cuab yeej zoo yog thawj kauj ruam.Xaiv lub microplate tsim nyog yuav pab tau qhov kev sim ua tiav.
Cov khoom siv ntawmELISAphaj feem ntau yog polystyrene (PS), thiab polystyrene muaj cov tshuaj tsis zoo thiab muaj peev xwm yaj tau los ntawm ntau yam organic solvents (xws li aromatic hydrocarbons, halogenated hydrocarbons, thiab lwm yam), thiab tuaj yeem corroded los ntawm muaj zog acids thiab alkalis.Tsis resistant rau roj thiab yooj yim discolored tom qab raug UV teeb.
Yam li casELISAdaim hlau puas muaj?
✦ Xaiv los ntawm xim
Pob tshab phaj:haum rau ntau thiab zoo-theem immunoassays thiab khi kev soj ntsuam;
Dawb phaj:haum rau tus kheej luminescence thiab chemiluminescence;
Dub phaj:haum rau fluorescent immunoassays thiab khi kev soj ntsuam.
✦ Xaiv los ntawm kev khi lub zog
Low-binding phaj:Passively khi rau cov proteins los ntawm qhov chaw hydrophobic bonds.Nws yog qhov tsim nyog raws li cov khoom thauj khoom siv rau macromolecular proteins nrog qhov hnyav molecular> 20kD.Nws muaj peev xwm sib khi cov protein yog 200 ~ 300ng IgG / cm2.
High binding phaj:Tom qab kev kho mob saum npoo, nws lub peev xwm ntawm cov protein khi tau zoo heev, ncav cuag 300 ~ 400ng IgG / cm2, thiab qhov hnyav molecular ntawm cov protein tseem ceeb yog> 10kD.
✦Txiav los ntawm cov duab hauv qab
Plat hauv qab:qis refractive index, haum rau kev tshawb nrhiav nrog microplate nyeem ntawv;
U hauv qab:Lub refractive index yog siab, uas yog yooj yim rau kev ntxiv, aspirating, sib tov thiab lwm yam hauj lwm.Koj tuaj yeem saib ncaj qha cov xim hloov los ntawm kev tshuaj xyuas qhov muag yam tsis tas muab tso rau ntawm tus nyeem ntawv microplate los txiav txim seb puas muaj cov tshuaj tiv thaiv kab mob sib raug.
Post lub sij hawm: Dec-22-2023