1. Cov cuab yeej siv
1. Cov khoom siv hauv chav npaj
Ib qho distilled dej distiller, ob chav distilled dej distiller, kua qaub tank, qhov cub, siab cooker, cia txee (kwv yees unsterilized khoom), cia txee ( khaws cia sterilized khoom), ntim rooj.Cov khoom siv hauv chav npaj kev daws teeb meem: torsion tshuav nyiaj li cas thiab hluav taws xob sib npaug (hnyav tshuaj), PH meter ( ntsuas PH tus nqi ntawm kab lis kev cai daws teeb meem), sib nqus stirrer (configuring tov chav rau do tov).
2. Cov khoom siv ntawm chav kab lis kev cai
Cov kua nitrogen tank, lub txee cia (khoom noj khoom haus), fluorescent teeb thiab ultraviolet teeb, cua purifier system, txias txias txias (- 80 ℃), cua txias, carbon dioxide lub tog raj kheej, sab rooj (sau cov ntaub ntawv xeem).
3. Cov khoom siv uas yuav tsum tau muab tso rau hauv chav tsis muaj menyuam
Centrifuge (collecting cells), ultra-clean worktable, inverted microscope, CO2 incubator (incubating culture), water da dej, peb-oxygen disinfection thiab sterilization tshuab, 4 ℃ tub yees (tso kua tshuaj thiab kab lis kev cai).
2 、 Kev ua haujlwm aseptic
(1) Sterilization ntawm chav tsis muaj menyuam
1. Ntxuav chav tsis tu ncua: ib zaug ib lub lim tiam, thawj zaug siv cov kais dej los ntxuav hauv pem teb, so lub rooj, thiab ntxuav lub rooj ua haujlwm, thiab tom qab ntawd siv 3 ‰ lysol los yog bromogeramine los yog 0.5% peracetic acid los so.
2. Sterilization ntawm CO2 incubator (incubator): ua ntej so nrog 3 ‰ bromogeramine, ces so nrog 75% cawv los yog 0.5% peracetic acid, thiab ces irradiate nrog ultraviolet teeb.
3. Kev ua kom tsis muaj menyuam ua ntej kev sim: tig rau lub teeb ultraviolet, peb lub tshuab pa oxygen thiab lub tshuab cua purifier rau 20-30 feeb raws li.
4. Kev ua kom tsis muaj menyuam tom qab qhov kev sim: so lub rooj ntxuav ultra-ntxuav, sab lub rooj thiab lub tshuab tsom iav inverted nrog 75% cawv (3 ‰ bromogeramine).
Sterilization npaj cov neeg ua haujlwm kuaj
1. Ntxuav tes nrog xab npum.
2. Hnav cov khaub ncaws cais, cais lub kaus mom, lub qhov ncauj qhov ntswg thiab khau khiab.
3. So tes nrog 75% cawv paj rwb pob.
Ua qauv qhia kev ua haujlwm tsis muaj menyuam
1. Txhua lub raj mis cawv, PBS, kab lis kev cai nruab nrab thiab trypsin coj mus rau hauv lub workbench ultra-huv yuav tsum tau so nrog 75% cawv nyob rau sab nrauv ntawm lub raj mis.
2. Ua haujlwm ze ntawm cov nplaim dej cawv.
3. Cov tais diav yuav tsum tau ua kom tsis muaj menyuam ua ntej siv.
4. Cov khoom siv (xws li lub kaus mom lub raj mis thiab cov raj xa dej) uas txuas ntxiv siv yuav tsum tau muab tso rau ntawm qhov chaw siab, thiab tseem yuav tsum tau kub dhau thaum siv.
5. Txhua txoj haujlwm yuav tsum nyob ze ntawm lub teeb cawv, thiab qhov kev txiav txim yuav tsum yog lub teeb thiab raug, thiab yuav tsum tsis txhob kov random.Yog tias cov quav cab tsis tuaj yeem kov cov kua dej khib nyiab.
6. Thaum aspirating ntau tshaj ob hom kua, ua tib zoo hloov cov yeeb nkab nqus kom tiv thaiv kev sib kis.
Saib tshooj tom ntej rau kev tua kab mob.
Post lub sij hawm: Feb-01-2023