Polymerase chain reaction, abbreviated asPCRhauv lus Askiv, yog cov txheej txheem molecular biology siv los nthuav cov DNA tshwj xeeb.Nws tuaj yeem suav tau tias yog qhov tshwj xeeb DNA replication sab nraum lub cev, uas tuaj yeem ua rau muaj qhov tsawg heev ntawm DNA.Thaum tag nrhoPCRCov txheej txheem tshuaj tiv thaiv, ib chav kawm ntawm cov tshuaj plays lub luag haujlwm tseem ceeb - enzymes.
1. TAQ DNA
Nyob rau hauv kev sim nyob rau hauv thaum ntxov hnub ntawmPCR, cov kws tshawb fawb tau siv Escherichia coli DNA polymerase I, Tab sis muaj ib qho teeb meem nrog cov enzyme no: nws yuav tsum tau ntxiv cov enzyme tshiab txhua lub sijhawm ua haujlwm, uas ua rau cov kauj ruam ua haujlwm nyuaj me ntsis thiab nws nyuaj rau kev ua kom zoo dua qub.Qhov teeb meem no tau daws tau tom qab cov kws tshawb fawb yuam kev cais tawm Taq DNA polymerase los ntawm Thermus aquaticus xyoo 1988. Txij thaum ntawd los, qhov tsis siv neeg amplification ntawm DNA tau dhau los ua qhov tseeb.Kev tshawb pom ntawm cov enzyme no kuj ua rauPCRtechnology yooj yim, tswv yim thiab universal technology.Tam sim no, Taq DNA polymerase yog cov polymerase ntau tshaj plaws hauv cov khoom siv DNA.
2. PfuDNA
Raws li tau hais los saum toj no, Taq DNase muaj cov kab mob loj, yog li cov kws tshawb fawb tau hloov kho Taq DNA polymerase rau qee yam txhawm rau zam kom tsis txhob muaj qhov txawv txav vim tsis sib xws, ua rau cov txiaj ntsig tsis raug.Tab sis kev hloov kho ntawm Taq DNA polymerase tuaj yeem cuam tshuam cov haujlwm ntawm DNA polymerase ntawm chav tsev kub.PfuDNA polymerase tuaj yeem ua tau zoo rau cov teeb meem saum toj no ntawm Taq DNA polymerase, kom cov tshuaj tiv thaiv PCR tuaj yeem ua tiav ib txwm, thiab qhov kev vam meej ntawm lub hom phiaj gene amplification tuaj yeem txhim kho tau zoo.
3. Rov qab cov ntaub ntawv rov qab
Rov qab transcriptase tau tshawb pom nyob rau xyoo 1970. Qhov enzyme no siv RNA ua qauv, dNTP ua lub substrate, ua raws li lub hauv paus ntawm kev sib koom ua ke, thiab sib txuas ua ke DNA ib txoj hlua sib txuas ntxiv rau RNA template hauv 5′-3′ kev taw qhia.Rov qab transcriptase feem ntau yog nyob ntawm DNA polymerase kev ua los ntawm DNA lossis RNA cov qauv thiab yog li tsis muaj 3′-5′ exonuclease kev ua.Txawm li cas los xij, nws muaj RNase H kev ua haujlwm, uas txwv qhov kev sib txuas ntev ntawm kev rov qab transcriptase rau qee yam.Vim tsis muaj kev ncaj ncees thiab kev kub ntxhov ntawm cov tsiaj qus rov qab transcriptase, cov kws tshawb fawb kuj tau hloov kho nws.
RauPCRCov kev sim, cov khoom siv tseem ceeb yog: ib leeg PCR raj, 4/8-strip PCR raj, PCR daim hlau.
Labio uaPCR khoom sivmuaj cov hauv qab noqhov zoo:
PCR daim hlau: Broad thermal cycler compatibility;High-contrast, yooj yim zoo qhia;zoo fluorescence reflection; zoohloov tshav kub;Certified DNase, RNase, DNA, PCR inhibitors, thiab kuaj tsis muaj pyrogen.
Ib leeg PCR raj: Evaporation-resistant; zoohloov tshav kub;zoo heev optical clarity; Certified DNase, RNase, DNA, PCR inhibitors, thiab kuaj pyrogen-dawb.
4/8-strips PCR raj: Ultra-nyias phab ntsa; siab clarity; zoo fluorescence reflection;tuaj yeem siv hauv kev lag luam tshuaj, kev lag luam zaub mov, thiab molecular biology; cov khoom zoo, cov khoom siv PP; Certified DNase, RNase, DNA, PCR inhibitors, thiab kuaj tsis muaj pyrogen.
Post lub sij hawm: Jun-09-2023