Cov kab lis kev cai ntawm tes tuaj yeem muab faib ua lub tiaj tiaj hauv qab thiab puag ncig hauv qab (U-shaped thiab V-shaped) raws li cov duab hauv qab;Tus naj npawb ntawm kab lis kev cai yog 6, 12, 24, 48, 96, 384, 1536, thiab lwm yam;Raws li cov ntaub ntawv sib txawv, muaj Terasaki phaj thiab cov kab lis kev cai ntawm tes zoo tib yam.Cov kev xaiv tshwj xeeb yog nyob ntawm hom kab lis kev cai, qhov xav tau ntawm kab lis kev cai ntim thiab cov hom phiaj sib txawv.
(1) Qhov sib txawv thiab xaiv ntawm lub tiaj thiab puag ncig hauv qab (U-shaped thiab V-shaped) kab lis kev cai daim hlau
Cov duab sib txawv ntawm kab lis kev cai daim hlau muaj kev siv sib txawv.Cov kab lis kev cai kab lis kev cai feem ntau yog tiaj tus hauv qab, uas yooj yim rau kev soj ntsuam microscopic, nrog thaj chaw ntshiab hauv qab thiab sib npaug ntawm cov kab lis kev cai ntawm cov kua dej.Yog li ntawd, thaum ua MTT thiab lwm yam kev sim, cov phaj hauv qab yog feem ntau siv, tsis hais seb cov cell txuas nrog phab ntsa lossis raug tshem tawm.Lub tiaj hauv qab kab lis kev cai phaj yuav tsum tau siv los ntsuas tus nqi absorbance.Ua tib zoo saib xyuas cov khoom siv, thiab kos "Tissue Culture (TC) Treated" rau kab lis kev cai ntawm tes.
U-shaped lossis V-shaped daim hlau feem ntau yog siv rau qee qhov tshwj xeeb.Piv txwv li, hauv kev tiv thaiv kab mob, thaum ob hom lymphocytes sib xyaw ua ke rau kab lis kev cai, lawv yuav tsum sib cuag thiab txhawb nqa ib leeg.Lub sijhawm no, U-shaped daim hlau feem ntau yog siv vim tias cov hlwb yuav sib sau ua ke hauv ib qho me me vim qhov cuam tshuam ntawm lub ntiajteb txawj nqus.Cov kab lis kev cai puag ncig hauv qab kuj tuaj yeem siv rau kev sim ntawm isotope incorporation, uas yuav tsum tau cov khoom siv ntawm tes los sau cov kab lis kev cai ntawm tes, xws li "cov kab lis kev cai sib xyaw lymphocyte".V-shaped daim hlau feem ntau yog siv rau kev tua cov xov tooj ntawm tes thiab kev kuaj ntshav agglutination.Kev sim ntawm kev tua ntawm tes kuj tuaj yeem hloov tau los ntawm U-shaped phaj (tom qab ntxiv cov hlwb, centrifuge ntawm qis ceev).
(2) Qhov sib txawv ntawm Terasaki phaj thiab zoo tib yam cell kab lis kev cai phaj
Terasaki phaj feem ntau yog siv rau kev tshawb fawb crystallographic.Cov khoom tsim yog yooj yim rau kev soj ntsuam siv lead ua thiab kev tsom xam cov qauv.Muaj ob txoj hauv kev: zaum thiab dai poob.Ob txoj kev siv cov khoom sib txawv configuration.Crystal class polymer yog xaiv raws li cov khoom siv, thiab cov ntaub ntawv tshwj xeeb yog qhov zoo rau kev soj ntsuam cov qauv siv lead ua.
Lub cell kab lis kev cai phaj yog tsuas yog ua los ntawm PS cov khoom, thiab cov khoom yog kho nto, uas yog yooj yim rau cell adherent kev loj hlob thiab extension.Tau kawg, kuj tseem muaj cov ntaub ntawv loj hlob ntawm cov hlwb planktonic, nrog rau qhov sib khi qis.
(3) Qhov sib txawv ntawm cell kab lis kev cai phaj thiab Elisa phaj
Elisa phaj feem ntau kim dua li cov kab lis kev cai ntawm tes.Lub cell phaj yog tsuas yog siv rau kab lis kev cai ntawm tes thiab tseem tuaj yeem siv los ntsuas cov protein ntau;Lub phaj Elisa suav nrog cov phaj txheej thiab cov tshuaj tiv thaiv phaj, thiab feem ntau tsis tas yuav siv rau cov kab lis kev cai ntawm tes.Nws yog tsuas yog siv rau kev kuaj pom cov protein tom qab lub cev tiv thaiv kab mob enzyme-txuas cov tshuaj tiv thaiv, xav tau cov kev xav tau siab dua thiab tshwj xeeb enzyme daim ntawv lo ua haujlwm daws teeb meem.
(4) Qhov chaw hauv qab thiab pom zoo kom siv cov kua ntau ntawm cov kab lis kev cai sib txawv
Cov kua theem ntawm cov kab lis kev cai ua kua ntxiv rau cov ntawv sib txawv ntawm qhov chaw yuav tsum tsis txhob sib sib zog nqus, feem ntau nyob rau hauv thaj tsam ntawm 2 ~ 3 hli.Cov kua dej tsim nyog ntawm txhua qhov kab lis kev cai tuaj yeem suav tau los ntawm kev sib txuas hauv qab ntawm qhov sib txawv.Yog tias cov kua ntau ntxiv, cov pa roj (oxygen) pauv yuav raug cuam tshuam, thiab nws yooj yim dhau los ntawm kev txav mus los, ua rau muaj kuab paug.Qhov tshwj xeeb cell ceev nyob ntawm lub hom phiaj ntawm qhov kev sim.
Post lub sij hawm: Nov-04-2022