Cov qauv sau, khaws cia thiab kev thauj mus los rau cov kev sim ntau

Cov qauv sau, khaws cia thiab kev thauj mus los rau cov kev sim ntau

1. Kev sau thiab khaws cia ntawm cov kab mob pathological:

☛ Frozen seem: Tshem tawm cov ntaub so ntswg tsim nyog thiab khaws cia rau hauv cov kua nitrogen;

☛ Paraffin sectioning: Tshem cov ntaub so ntswg tsim nyog thiab khaws cia rau hauv 4% paraformaldehyde;

☛ Cell slides: Cell slides tau kho nyob rau hauv 4% paraformaldehyde rau 30 feeb, ces hloov nrog PBS thiab raus hauv PBS thiab khaws cia ntawm 4 ° C.

2. Kev sau thiab khaws cia ntawm molecular biology specimens:

☛ Cov ntaub so ntswg tshiab: Txiav cov qauv thiab muab tso rau hauv cov kua nitrogen los yog -80 ° C lub tub yees;

☛ Paraffin specimens: Khaws ntawm chav sov;

☛ Tag nrho cov ntshav kuaj: Siv cov ntshav kom tsim nyog thiab ntxiv EDTA lossis heparin anticoagulation ntshav sau raj;

☛ Cov kua dej hauv lub cev: kev kub ceev centrifugation los sau cov av;

☛Cell specimens: Cells yog lysed nrog TRizol thiab muab cia rau hauv cov kua nitrogen los yog -80 ° C tub yees.

3. Kev sau thiab khaws cia cov qauv kuaj protein:

☛ Cov ntaub so ntswg tshiab: Txiav cov qauv thiab muab tso rau hauv cov kua nitrogen los yog -80 ° C lub tub yees;

☛ Tag nrho cov ntshav kuaj: Siv cov ntshav kom tsim nyog thiab ntxiv EDTA lossis heparin anticoagulation ntshav sau raj;

☛Cell specimens: Cells yog tag nrho lysed nrog cell lysis tov thiab ces muab cia rau hauv kua nitrogen los yog -80 ° C tub yees.

4. Kev sau thiab khaws cia ntawm ELISA, xov tooj cua immunoassay, thiab biochemical sim cov qauv:

☛ Cov kua dej (plasma) qauv: Muab cov ntshav tag nrho thiab ntxiv rau hauv ib lub raj procoagulation (anticoagulation tube), centrifuge ntawm 2500 rpm rau li 20 feeb, sau cov supernatant, thiab muab cia rau hauv cov kua nitrogen los yog hauv -80 ° C lub tub yees;

☛ Cov qauv tso zis: centrifuge tus qauv ntawm 2500 rpm li 20 feeb, thiab muab cia rau hauv cov kua nitrogen los yog -80 ° C lub tub yees;xa mus rau txoj kev no rau cov kua dej thoracic thiab ascites, cerebrospinal kua, thiab alveolar lavage kua;

☛ Cov qauv ntawm tes: Thaum kuaj pom cov khoom zais cia, centrifuge cov qauv ntawm 2500 rpm li 20 feeb thiab muab cia rau hauv cov kua nitrogen lossis -80 ° C lub tub yees;Thaum kuaj pom cov khoom siv hauv lub cev, Dilute lub cell ncua kev kawm nrog PBS thiab khov thiab thaw ntau zaus los rhuav tshem cov hlwb thiab tso tawm cov khoom hauv lub cev.centrifuge ntawm 2500 rpm li 20 feeb thiab sau cov supernatant raws li saum toj no;

☛ Cov qauv ntaub so ntswg: Tom qab txiav cov qauv, ntsuas lawv thiab khov rau hauv cov kua nitrogen lossis -80 ° C lub tub yees rau siv tom qab.

5. Metabolomics specimen collection:

☛ Cov qauv tso zis: centrifuge tus qauv ntawm 2500 rpm li 20 feeb thiab muab tso rau hauv cov kua nitrogen los yog -80 ° C tub yees;xa mus rau txoj kev no rau cov kua dej thoracic thiab ascites, cerebrospinal kua, alveolar lavage kua, thiab lwm yam.;

☛ Tom qab txiav cov qauv ntaub so ntswg, ntsuas nws thiab khov nws hauv cov kua nitrogen lossis -80 ° C lub tub yees rau siv tom qab;