Kev ceev faj rau kev siv Petri tais diav
Kev ntxuav ntawm Petri tais diav
1. Soaking: Soak tshiab los yog siv khob iav nrog dej huv kom soften thiab dissolve qhov txuas.Ua ntej siv cov iav tshiab, tsuas yog txhuam nws nrog cov kais dej, thiab tom qab ntawd tsau nws hauv 5% hydrochloric acid thaum hmo ntuj;Cov khoom siv iav feem ntau muaj ntau cov protein thiab roj, uas tsis yooj yim rau txhuam tawm tom qab ziab, yog li nws yuav tsum tau muab tso rau hauv dej huv tam sim ntawd tom qab siv txhuam hniav.
2. Txhuam Txhuam: muab cov khob iav ntim rau hauv cov dej xab npum thiab txhuam nrog txhuam txhuam.Tsis txhob tawm ntawm qhov chaw tuag thiab tiv thaiv kev puas tsuaj rau qhov chaw tiav ntawm cov ntim.Ntxuav thiab qhuav cov iav ntxuav kom qhuav.
3. Pickling: Pickling yog tsau cov hlab ntsha saum toj no rau hauv cov tshuaj ntxuav, tseem hu ua kua qaub, kom tshem tawm cov khoom seem ntawm cov hlab ntsha los ntawm qhov muaj zog oxidation ntawm cov kua qaub.Pickling yuav tsum tsis pub tsawg tshaj li rau teev, feem ntau thaum hmo ntuj lossis ntev dua.Ceev faj thaum tso thiab noj cov tais diav.
4. Flushing: Cov hlab ntsha tom qab txhuam thiab pickling yuav tsum tau yaug nrog dej.Txawm hais tias cov hlab ntsha raug ntxuav kom huv tom qab pickling ncaj qha cuam tshuam rau kev vam meej lossis tsis ua tiav ntawm kab lis kev cai ntawm tes.Rau phau ntawv ntxuav cov hlab ntsha, txhua lub nkoj yuav tsum tau "sau nrog dej - emptied" rau tsawg kawg 15 zaug, thiab thaum kawg ntxuav rau 2-3 zaug nrog re steaming dej, qhuav los yog qhuav, thiab ntim rau standby.
5. Cov kab lis kev cai yas pov tseg feem ntau yog rays sterilized los yog chemically sterilized thaum lawv tawm hauv lub hoobkas.
Classification ntawm Petri tais diav
2. Nws tuaj yeem muab faib ua cov yas petri tais diav thiab iav petri tais diav raws li cov khoom siv sib txawv, tab sis ob qho tib si tuaj txawv teb chaws petri tais diav thiab pov tseg petri tais diav yog cov ntaub ntawv yas.
3. Raws li qhov ntau thiab tsawg, lawv tuaj yeem muab faib ua 35mm, 60mm thiab 90mm inch.150mm Petri phaj.
4. Raws li qhov sib txawv, nws tuaj yeem muab faib ua 2 cais Petri tais diav, 3 cais Petri tais diav, thiab lwm yam.
5. Cov ntaub ntawv ntawm kab lis kev cai tais diav yog muab faib ua ob pawg, feem ntau yog yas thiab iav.Cov iav tuaj yeem siv rau cov khoom siv cog, kab lis kev cai microbial thiab kab lis kev cai ntawm cov tsiaj hlwb.Cov ntaub ntawv yas yuav yog cov khoom siv polyethylene, uas tuaj yeem siv tau ib zaug lossis ntau zaus.Lawv tsim nyog rau kev kuaj inoculation, scribing, thiab cov kab mob sib cais, thiab tuaj yeem siv rau kev cog qoob loo ntawm cov khoom cog.
Kev ceev faj rau kev siv Petri tais diav
1. Cov kab lis kev cai tais diav yog ntxuav thiab tua kab mob ua ntej siv.Txawm hais tias nws huv los yog tsis muaj kev cuam tshuam zoo rau kev ua haujlwm, uas tuaj yeem cuam tshuam rau pH ntawm kab lis kev cai nruab nrab.Yog tias qee cov tshuaj muaj nyob, nws yuav inhibit qhov kev loj hlob ntawm cov kab mob.
2. Cov kab lis kev cai tshiab yuav tsum tau ntxuav nrog dej kub ua ntej, ces muab tso rau hauv 1% lossis 2% hydrochloric acid tov rau ob peb teev kom tshem tawm cov tshuaj dawb alkaline, thiab tom qab ntawd ntxuav ob zaug nrog dej distilled.
3. Txhawm rau cog cov kab mob, siv lub zog siab siab (feem ntau 6.8 * 10 Pa siab siab chav rau 5 lub zog), sterilize rau 30min ntawm 120 ℃, qhuav hauv chav tsev kub, lossis siv cov cua sov kom tsis muaj menyuam, uas yog, muab cov kab lis kev cai tais tso rau hauv qhov cub, khaws cia rau 2h ntawm 120 ℃, thiab tom qab ntawd tua cov kab mob hniav.
4. Sterilized kab lis kev cai tais diav yuav siv tau rau inoculation thiab cultivation.
Post lub sij hawm: Dec-28-2022