Yuav siv cov raj cryopreservation li cas rau kev tshawb fawb thiab raug
Kev siv lub raj cryopreservation yog kev tshawb fawb, tsis yog ib qho yooj yim trilogy xws li qhib lub tank kua nitrogen, muab tso rau hauv lub raj cryopreservation thiab kaw cov kua nitrogen tank.Kev tshawb fawb thiab kev siv cov raj cryopreservation kom raug tuaj yeem zam qhov poob ntawm cov qauv thiab tiv thaiv kev nyab xeeb ntawm cov neeg sim.
Kev siv lub raj cryopreservation yog kev tshawb fawb, tsis yog ib qho yooj yim trilogy xws li qhib lub tank kua nitrogen, muab tso rau hauv lub raj cryopreservation thiab kaw cov kua nitrogen tank.Kev tshawb fawb thiab kev siv cov raj cryopreservation kom raug tuaj yeem zam qhov poob ntawm cov qauv thiab tiv thaiv kev nyab xeeb ntawm cov neeg sim.
Freezing tube: khov cov kauj ruam
Ntxuav cov hlwb nrog preheated PBS tov, nqus cov tshuaj, thiab npog lub hlwb nrog cov tshuaj uas muaj trypsin thiab EDTA (nyias kua txheej yog txaus, thiab cov concentration ntawm trypsin thiab EDTA yuav tsum tau txiav txim raws li lub cell kab).
Incubate hlwb ntawm 37 ℃ rau 3-5 feeb.
Tom qab cov cell tshem tawm hauv qab, qhov incubation yog txiav, nruab nrab uas muaj cov ntshav yog ntxiv, thiab cov hlwb yog maj mam muab tshem tawm nrog ib tug pipette.
Centrifuge lub cell ncua kev kawm ntawv (500 xg, 5 min) thiab resuspension nrog cov nruab nrab uas muaj cov ntshav.
Cell suav.
Centrifuge lub cell ncua kev kawm ntawv (500 xg, 5 feeb), tshem tawm cov supernatant, thiab resuspension lub hlwb nrog nruab nrab uas muaj cov kuab tshuaj uas tsim nyog ntim.
Sib tov cov hlwb thiab cov tshuaj cryopreservation (60% nruab nrab, 20% fetal bovine serum, 20% DMSO) hauv 1: 1 ntim piv, thiab tom qab ntawd hloov mus rau Cryo STM cryopreservation raj.Qhov ceev ntawm cov hlwb khov yog 1-5 × 106 pieces / ml.
Cryo uas muaj cov hlwb STM cryopreservation tube raug pom zoo kom txias ntawm tus nqi ntawm − 1 K / min, thiab lub raj cryopreservation tuaj yeem muab tso rau hauv ib lub thawv uas muaj isopropanol ntawm -70 ℃.Yog tias Cryo STM cryopreservation tube khaws lwm cov qauv, uas tuaj yeem muab tso ncaj qha rau ntawm − 20 ℃, − 70 ℃ lossis theem roj ntawm cov kua nitrogen.Txhawm rau xyuas kom meej tias cov qauv yog khov tsis sib xws, 4 mL thiab 5 mL Cryo Lub sTM cryopreservation raj yuav tsum tau muab tso rau hauv lub tub yees ntawm − 20 ℃ rau ib hmos, thiab tom qab ntawd xa mus rau theem roj ntawm − 70 ℃ lossis kua nitrogen.
Tom qab ntawd hloov Cryo.sTM cryopreservation raj rau kua nitrogen tank.Txhawm rau kom tsis txhob muaj kuab paug (xws li mycoplasma) thiab kev saib xyuas kev nyab xeeb, thov tso Cryo.sTM cryopreservation tube nyob rau hauv cov roj theem ntawm cov kua nitrogen, tsis yog nyob rau hauv cov kua theem.
Yuav siv cov raj cryopreservation li cas rau kev tshawb fawb thiab raug?Peb lub tuam txhab yog tsim los ntawm cov kws tshaj lij nrog kev lag luam keeb kwm yav dhau los thiab kev lag luam nplua nuj los muab cov khoom lag luam rau kev tshawb fawb txog kev ua neej nyob thiab kev pabcuam rau cov kws tshawb fawb tshawb fawb.Nws tuaj yeem tsis tsuas yog ua tau raws li cov kev cai tshwj xeeb ntawm R & D cov neeg siv khoom ntawm cov khoom lag luam thiab ntim khoom, tab sis kuj ua tau raws li cov kev xav tau ntawm cov tuam txhab tsim khoom ntawm txhua theem los ntawm cov nplai me me, nruab nrab rau cov khoom loj.
Post lub sij hawm: Nov-25-2022