Lub raj centrifuge yog ib lub raj yooj yim uas tuaj yeem tiv taus kev sib hloov ceev thiab siab, xws li cais qee cov qauv thiab sib cais cov sediments supernatant.Ultrafiltration centrifuge raj muaj ob qhov zoo ib yam li lub raj sab hauv thiab sab nraud.Lub raj sab hauv yog ib daim nyias nyias uas muaj qhov hnyav molecular.Thaum lub sij hawm kev kub ceev centrifugation, cov neeg uas muaj lub cev me me ntawm qhov hnyav yuav nkag mus rau hauv lub raj qis (piv txwv li lub raj sab nraud), thiab cov uas muaj qhov hnyav dua molecular yuav raug kaw rau hauv lub raj sab sauv (xws li lub raj sab hauv).Qhov no yog lub hauv paus ntsiab lus ntawm ultrafiltration thiab feem ntau yog siv los mloog cov qauv.
Ultrafiltration centrifuge raj feem ntau tuaj yeem siv yam tsis muaj kev kho mob ua ntej, tab sis rau cov qauv ua qauv, tshwj xeeb tshaj yog rau dilute protein daws (< 10ug / ml), qhov rov qab ntawm cov concentration nrog ultrafiltration membranes feem ntau tsis muaj ntau.Txawm hais tias PES cov ntaub ntawv txo qis cov adsorption tsis tshwj xeeb, qee cov proteins, tshwj xeeb tshaj yog thaum lawv dilute, tej zaum yuav muaj teeb meem.Cov degree ntawm nonspecific binding sib txawv nrog cov qauv ntawm ib tug neeg cov proteins.Cov protein uas muaj cov nqi them los yog cov dej hydrophobic feem ntau yuav khi irreversibly rau ntau qhov chaw.Passivation pretreatment rau saum npoo ntawm ultrafiltration centrifuge raj tuaj yeem txo qhov poob ntawm cov protein adsorption ntawm daim nyias nyias.Feem ntau, pretreatment ntawm kem ua ntej concentrating dilute protein tov tuaj yeem txhim kho tus nqi rov qab, vim hais tias cov tshuaj muaj peev xwm ua kom tiav cov protein adsorption qhov chaw tawm ntawm daim nyias nyias thiab nto.Txoj kev passivation yog ua ntej soak hauv kem nrog lub ntim ntau dua ntawm cov tshuaj passivation rau ntau tshaj 1 teev, ntxuav lub kem kom huv si nrog dej distilled, thiab ces centrifuge nws ib zaug nrog dej distilled kom tshem tawm tag nrho cov passivation tov uas yuav nyob twj ywm rau hauv zaj duab xis. .Ceev faj tsis txhob cia zaj duab xis qhuav tom qab passivation.Yog tias koj xav siv nws tom qab, koj yuav tsum tau ntxiv cov dej tsis huv los ua kom cov yeeb yaj kiab moist.
Ultrafiltration centrifuge tubes feem ntau tsis tuaj yeem ua kom tsis muaj menyuam thiab rov siv dua.Txij li thaum tus nqi ntawm ib lub raj tsis pheej yig, ntau tus neeg sim rov qab siv nws - qhov kev paub yog los ntxuav cov membrane nto nrog dej distilled rau ntau zaus thiab centrifuge nws ib zaug lossis ob zaug.Lub raj me me uas tuaj yeem centrifuged rov qab tuaj yeem muab tso rau hauv dej distilled thiab tom qab ntawd centrifuged rov qab rau ntau lub sijhawm, uas yuav zoo dua.Nws tuaj yeem siv rau cov qauv qub, thiab tuaj yeem tsau rau hauv cov dej distilled thaum tsis siv, tab sis cov kab mob yuav tsum tau tiv thaiv.Tsis txhob muab cov qauv sib txawv.Qee tus neeg hais tias soaking hauv 20% cawv thiab 1n NaOH (sodium hydroxide) tuaj yeem tiv thaiv kev loj hlob ntawm cov kab mob thiab tiv thaiv kom qhuav.Ntev npaum li lub ultrafiltration membrane invades dej, nws tsis tuaj yeem tso cai kom qhuav.Txawm li cas los xij, lwm tus hais tias nws yuav rhuav tshem cov qauv membrane.Nyob rau hauv txhua rooj plaub, manufacturers feem ntau tsis txhawb rov siv dua.Kev siv rov ua dua yuav thaiv qhov pore loj ntawm lub lim membrane, thiab tseem ua rau cov kua xau, uas yuav cuam tshuam rau qhov kev sim.
Post lub sij hawm: Sep-05-2022